Identification of an AU-rich translational enhancer within the Escherichia coli fepB leader RNA.

ABSTRACT: The fepB gene encodes a periplasmic binding protein that is essential for the uptake of ferric enterobactin by Escherichia coli. Its transcription is regulated in response to iron levels by the Fur repressor. The fepB transcript includes a 217-nucleotide leader sequence with several features suggestive of posttranscriptional regulation. To investigate the fepB leader for its contribution to fepB expression, defined deletions and substitution mutations in the leader were characterized using fepB-phoA translational fusions. The fepB leader was found to be necessary for maximal fepB expression, primarily due to the influence of an AU-rich translational enhancer (TE) located 5' to the Shine-Dalgarno sequence. Deletions or substitutions within the TE sequence decreased fepB-phoA expression five

SUBMITTER: Hook-Barnard IG 

PROVIDER: S-EPMC1913407 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature