Transcript-level annotation of Affymetrix probesets improves the interpretation of gene expression data.

ABSTRACT:

Background

The wide use of Affymetrix microarray in broadened fields of biological research has made the probeset annotation an important issue. Standard Affymetrix probeset annotation is at gene level, i.e. a probeset is precisely linked to a gene, and probeset intensity is interpreted as gene expression. The increased knowledge that one gene may have multiple transcript variants clearly brings up the necessity of updating this gene-level annotation to a refined transcript-level.

Results

Through performing rigorous alignments of the Affymetrix probe sequences against a comprehensive pool of currently available transcript sequences, and further linking the probesets to the International Protein Index, we generated transcript-level or protein-level annotation tables for two p

SUBMITTER: Yu H 

PROVIDER: S-EPMC1913542 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature