Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L.

ABSTRACT:

Background

Somatic embryogenesis (SE) is an asexual propagation pathway requiring a somatic-to-embryonic transition of differentiated somatic cells toward embryogenic cells capable of producing embryos in a process resembling zygotic embryogenesis. In chicory, genetic variability with respect to the formation of somatic embryos was detected between plants from a population of Cichorium intybus L. landrace Koospol. Though all plants from this population were self incompatible, we managed by repeated selfing to obtain a few seeds from one highly embryogenic (E) plant, K59. Among the plants grown from these seeds, one plant, C15, was found to be non-embryogenic (NE) under our SE-inducing conditions. Being closely related, we decided to exploit the difference in SE capacity between K59

SUBMITTER: Legrand S 

PROVIDER: S-EPMC1913917 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature