Mutational pattern and frequency of induced nucleotide changes in mouse ENU mutagenesis.

ABSTRACT:

Background

With the advent of sequence-based approaches in the mutagenesis studies, it is now possible to directly evaluate the genome-wide pattern of experimentally induced DNA sequence changes for a diverse array of organisms. To gain a more comprehensive understanding of the mutational bias inherent in mouse ENU mutagenesis, this study describes a detailed evaluation of the induced mutational pattern obtained from a sequence-based screen of ENU-mutagenized mice.

Results

Based on a large-scale screening data, we derive the sequence-based estimates of the nucleotide-specific pattern and frequency of ENU-induced base replacement mutation in the mouse germline, which are then combined with the pattern of codon usage in the mouse coding sequences to infer the spectrum of amino

SUBMITTER: Takahasi KR 

PROVIDER: S-EPMC1914352 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature