Homologous high-throughput expression and purification of highly conserved E coli proteins.

ABSTRACT:

Background

Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal intestinal flora is crucial for the development of intestinal inflammation. However, due to the complexity of the intestinal flora, it has been difficult to design experiments for detection of proinflammatory bacterial antigen(s) involved in the pathogenesis of the disease. Several studies indicated a potential association of E. coli with IBD. In addition, T cell clones of IBD patients were shown to cross react towards antigens from different enteric bacterial species and thus likely responded to conserved bacterial antigens. We therefore chose highly conserved E. coli proteins as cand

SUBMITTER: Ergin A 

PROVIDER: S-EPMC1914363 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature