Positive-feedback loops as a flexible biological module.

ABSTRACT:

Background

Bistability in genetic networks allows cells to remember past events and to make discrete decisions in response to graded signals. Bistable behavior can result from positive feedback, but feedback loops can have other roles in signal transduction as well.

Results

We introduced positive feedback into the budding-yeast pheromone response to convert it into a bistable system. In the presence of feedback, transient induction with high pheromone levels caused persistent pathway activation, whereas at lower levels a fraction of cells became persistently active but the rest inactivated completely. We also generated mutations that quantitatively tuned the basal and induced expression levels of the feedback promoter and showed that they qualitatively changed the behavior o

SUBMITTER: Ingolia NT 

PROVIDER: S-EPMC1914375 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature