The effect of amino acid deletions and substitutions in the longest loop of GFP.

ABSTRACT:

Background

The effect of single and multiple amino acid substitutions in the green fluorescent protein (GFP) from Aequorea victoria has been extensively explored, yielding several proteins of diverse spectral properties. However, the role of amino acid deletions in this protein -as with most proteins- is still unknown, due to the technical difficulties involved in generating combinatorial in-phase amino acid deletions on a target region.

Results

In this study, the region I129-L142 of superglo GFP (sgGFP), corresponding to the longest loop of the protein and located far away from the central chromophore, was subjected to a random amino acid deletion approach, employing an in-house recently developed mutagenesis method termed Codon-Based Random Deletion (COBARDE). Only two mut

SUBMITTER: Flores-Ramirez G 

PROVIDER: S-EPMC1919350 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature