Profiling the DNA-binding specificities of engineered Cys2His2 zinc finger domains using a rapid cell-based method.

ABSTRACT: The C2H2 zinc finger is the most commonly utilized framework for engineering DNA-binding domains with novel specificities. Many different selection strategies have been developed to identify individual fingers that possess a particular DNA-binding specificity from a randomized library. In these experiments, each finger is selected in the context of a constant finger framework that ensures the identification of clones with a desired specificity by properly positioning the randomized finger on the DNA template. Following a successful selection, multiple zinc-finger clones are typically recovered that share similarities in the sequences of their DNA-recognition helices. In principle, each of the clones isolated from a selection is a candidate for assembly into a larger multi-finger protein, b

SUBMITTER: Meng X 

PROVIDER: S-EPMC1920264 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature