A novel technique for measuring variations in DNA copy-number: competitive genomic polymerase chain reaction.

ABSTRACT:

Background

Changes in genomic copy number occur in many human diseases including cancer. Characterization of these changes is important for both basic understanding and diagnosis of these diseases. Microarrays have recently become the standard technique and are commercially available. However, it is useful to have an affordable technique to complement them.

Results

We describe a novel polymerase chain reaction (PCR)-based technique, termed competitive genomic PCR (CGP). The main characteristic of CGP is that different adaptors are added to the sample and control genomic DNAs after appropriate restriction enzyme digestion. These adaptor-supplemented DNAs are subjected to competitive PCR using an adaptor-primer and a locus-specific primer. The amplified products are then separ

SUBMITTER: Iwao-Koizumi K 

PROVIDER: S-EPMC1920520 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature