A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells.

ABSTRACT:

Background

Staphylococcus aureus, a leading cause of chronic or acute infections, is traditionally considered an extracellular pathogen despite repeated reports of S. aureus internalization by a variety of non-myeloid cells in vitro. This property potentially contributes to bacterial persistence, protection from antibiotics and evasion of immune defenses. Mechanisms contributing to internalization have been partly elucidated, but bacterial processes triggered intracellularly are largely unknown.

Results

We have developed an in vitro model using human lung epithelial cells that shows intracellular bacterial persistence for up to 2 weeks. Using an original approach we successfully collected and amplified low amounts of bacterial RNA recovered from infected eukaryotic cells. Tr

SUBMITTER: Garzoni C 

PROVIDER: S-EPMC1924023 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature