Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera.

ABSTRACT:

Background

Larval molting and metamorphosis are important physiological processes in the life cycle of the holometabolous insect. We used suppression subtractive hybridization (SSH) to identify genes differentially expressed during larval molting and metamorphosis.

Results

We performed SSH between tissues from a variety of developmental stages, including molting 5th and feeding 6th instar larvae, metamorphically committed and feeding 5th instar larvae, and feeding 5th instar and metamorphically committed larvae. One hundred expressed sequence tags (ESTs) were identified and included 73 putative genes with similarity to known genes, and 27 unknown ESTs. SSH results were further characterized by dot blot, Northern blot, and RT-PCR. The expression levels of eleven genes were fo

SUBMITTER: Dong DJ 

PROVIDER: S-EPMC1925068 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature