New data on robustness of gene expression signatures in leukemia: comparison of three distinct total RNA preparation procedures.

ABSTRACT:

Background

Microarray gene expression (MAGE) signatures allow insights into the transcriptional processes of leukemias and may evolve as a molecular diagnostic test. Introduction of MAGE into clinical practice of leukemia diagnosis will require comprehensive assessment of variation due to the methodologies. Here we systematically assessed the impact of three different total RNA isolation procedures on variation in expression data: method A: lysis of mononuclear cells, followed by lysate homogenization and RNA extraction; method B: organic solvent based RNA isolation, and method C: organic solvent based RNA isolation followed by purification.

Results

We analyzed 27 pediatric acute leukemias representing nine distinct subtypes and show that method A yields better RNA quality,

SUBMITTER: Campo Dell'Orto M 

PROVIDER: S-EPMC1925098 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature