A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae.

ABSTRACT: Analysis of the function of a particular gene product typically involves determining the expression profile of the gene, the subcellular location of the protein, and the phenotype of a null strain lacking the protein. Conditional alleles of the gene are often created as an additional tool. We have developed a multifunctional, transposon-based system that simultaneously generates constructs for all the above analyses and is suitable for mutagenesis of any given Saccharomyces cerevisiae gene. Depending on the transposon used, the yeast gene is fused to a coding region for beta-galactosidase or green fluorescent protein. Gene expression can therefore be monitored by chemical or fluorescence assays. The transposons create insertion mutations in the target gene, allowing phenotypic analysis. Th

SUBMITTER: Ross-Macdonald P 

PROVIDER: S-EPMC19279 | biostudies-literature | 1997 Jan

REPOSITORIES: biostudies-literature