Inhibition of Escherichia coli biofilm formation by self-assembled monolayers of functional alkanethiols on gold.

ABSTRACT: Bacterial biofilms cause serious problems, such as antibiotic resistance and medical device-related infections. To further understand bacterium-surface interactions and to develop efficient control strategies, self-assembled monolayers (SAMs) of alkanethiols presenting different functional groups on gold films were analyzed to determine their resistance to biofilm formation. Escherichia coli was labeled with green florescence protein, and its biofilm formation on SAM-modified surfaces was monitored by confocal laser scanning microscopy. The three-dimensional structures of biofilms were analyzed with the COMSTAT software to obtain information about biofilm thickness and surface coverage. SAMs presenting methyl, L-gulonamide (a sugar alcohol tethered with an amide bond), and tri(ethylene gly

SUBMITTER: Hou S 

PROVIDER: S-EPMC1932777 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature