A general framework for designing and validating oligomer-based DNA microarrays and its application to Clostridium acetobutylicum.

ABSTRACT: While DNA microarray analysis is widely accepted as an essential tool for modern biology, its use still eludes many researchers for several reasons, especially when microarrays are not commercially available. In that case, the design, construction, and use of microarrays for a sequenced organism constitute substantial, time-consuming, and expensive tasks. Recently, it has become possible to construct custom microarrays using industrial manufacturing processes, which offer several advantages, including speed of manufacturing, quality control, no up-front setup costs, and need-based microarray ordering. Here, we describe a strategy for designing and validating DNA microarrays manufactured using a commercial process. The 22K microarrays for the solvent producer Clostridium acetobutylicum ATCC

SUBMITTER: Paredes CJ 

PROVIDER: S-EPMC1932840 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature