Rapid escape of the dot/icm mutants of Legionella pneumophila into the cytosol of mammalian and protozoan cells.

ABSTRACT: The Legionella pneumophila-containing phagosome evades endocytic fusion and intercepts endoplasmic reticulum (ER)-to-Golgi vesicle traffic, which is believed to be mediated by the Dot/Icm type IV secretion system. Although phagosomes harboring dot/icm mutants are thought to mature through the endosomal-lysosomal pathway, colocalization studies with lysosomal markers have reported contradictory results. In addition, phagosomes harboring the dot/icm mutants do not interact with endocytosed materials, which is inconsistent with maturation of the phagosomes in the endosomal-lysosomal pathway. Using multiple strategies, we show that the dot/icm mutants defective in the Dot/Icm structural apparatus are unable to maintain the integrity of their phagosomes and escape into the cytoplasm within minu

SUBMITTER: Molmeret M 

PROVIDER: S-EPMC1932949 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature