A PCR-based method for detection and quantification of small RNAs.

ABSTRACT: Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

SUBMITTER: Ro S 

PROVIDER: S-EPMC1934510 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature