DNA bar coding and pyrosequencing to identify rare HIV drug resistance mutations.

ABSTRACT: Treatment of HIV-infected individuals with antiretroviral agents selects for drug-resistant mutants, resulting in frequent treatment failures. Although the major antiretroviral resistance mutations are routinely characterized by DNA sequencing, treatment failures are still common, probably in part because undetected rare resistance mutations facilitate viral escape. Here we combined DNA bar coding and massively parallel pyrosequencing to quantify rare drug resistance mutations. Using DNA bar coding, we were able to analyze seven viral populations in parallel, overall characterizing 118 093 sequence reads of average length 103 bp. Analysis of a control HIV mixture showed that resistance mutations present as 5% of the population could be readily detected without false positive calls. In thre

SUBMITTER: Hoffmann C 

PROVIDER: S-EPMC1934997 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature