Horizontally transferred genes in plant-parasitic nematodes: a high-throughput genomic approach.

ABSTRACT:

Background

Published accounts of horizontally acquired genes in plant-parasitic nematodes have not been the result of a specific search for gene transfer per se, but rather have emerged from characterization of individual genes. We present a method for a high-throughput genome screen for horizontally acquired genes, illustrated using expressed sequence tag (EST) data from three species of root-knot nematode, Meloidogyne species.

Results

Our approach identified the previously postulated horizontally transferred genes and revealed six new candidates. Screening was partially dependent on sequence quality, with more candidates identified from clustered sequences than from raw EST data. Computational and experimental methods verified the horizontal gene transfer candidates as bon

SUBMITTER: Scholl EH 

PROVIDER: S-EPMC193618 | biostudies-literature | 2003

REPOSITORIES: biostudies-literature