Dynein modifiers in C. elegans: light chains suppress conditional heavy chain mutants.

ABSTRACT: Cytoplasmic dynein is a microtubule-dependent motor protein that functions in mitotic cells during centrosome separation, metaphase chromosome congression, anaphase spindle elongation, and chromosome segregation. Dynein is also utilized during interphase for vesicle transport and organelle positioning. While numerous cellular processes require cytoplasmic dynein, the mechanisms that target and regulate this microtubule motor remain largely unknown. By screening a conditional Caenorhabditis elegans cytoplasmic dynein heavy chain mutant at a semipermissive temperature with a genome-wide RNA interference library to reduce gene functions, we have isolated and characterized twenty dynein-specific suppressor genes. When reduced in function, these genes suppress dynein mutants but not other condi

SUBMITTER: O'Rourke SM 

PROVIDER: S-EPMC1937013 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature