Highly restricted localization of RNA polymerase II within a locus control region of a tissue-specific chromatin domain.

ABSTRACT: RNA polymerase II (Pol II) can associate with regulatory elements far from promoters. For the murine beta-globin locus, Pol II binds the beta-globin locus control region (LCR) far upstream of the beta-globin promoters, independent of recruitment to and activation of the betamajor promoter. We describe here an analysis of where Pol II resides within the LCR, how it is recruited to the LCR, and the functional consequences of recruitment. High-resolution analysis of the distribution of Pol II revealed that Pol II binding within the LCR is restricted to the hypersensitive sites. Blocking elongation eliminated the synthesis of genic and extragenic transcripts and eliminated Pol II from the betamajor open reading frame. However, the elongation blockade did not redistribute Pol II at the hypersen

SUBMITTER: Johnson KD 

PROVIDER: S-EPMC193707 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature