High-resolution analysis of Drosophila heterochromatin organization using SuUR Su(var)3-9 double mutants.

ABSTRACT: The structural and functional analyses of heterochromatin are essential to understanding how heterochromatic genes are regulated and how centromeric chromatin is formed. Because the repetitive nature of heterochromatin hampers its genome analysis, new approaches need to be developed. Here, we describe how, in double mutants for Su(var)3-9 and SuUR genes encoding two structural proteins of heterochromatin, new banded heterochromatic segments appear in all polytene chromosomes due to the strong suppression of under-replication in pericentric regions. FISH on salivary gland polytene chromosomes from these double mutant larvae allows high resolution of heterochromatin mapping. In addition, immunostaining experiments with a set of antibodies against euchromatic and heterochromatic proteins reve

SUBMITTER: Andreyeva EN 

PROVIDER: S-EPMC1937550 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature