Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis.

ABSTRACT: DNA microarrays are an excellent potential tool for clinical microbiology, since this technology allows relatively rapid identification and characterization of microbial and viral pathogens. In the present study, an oligonucleotide microarray was developed and used for the analysis of thermophilic Campylobacter spp., the primary food-borne pathogen in the United States. We analyzed four Campylobacter species: Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis. Our assay relies on the PCR amplification of specific regions in five target genes (fur, glyA, cdtABC, ceuB-C, and fliY) as a first step, followed by microarray-based analysis of amplified DNAs. Alleles of two genes, fur and glyA, which are found in all tested thermophilic Campylobacter spp., were used for identification and

SUBMITTER: Volokhov D 

PROVIDER: S-EPMC193862 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature