Analysis of the immune-related transcriptome of a lophotrochozoan model, the marine annelid Platynereis dumerilii.

ABSTRACT:

Background

The marine annelid Platynereis dumerilii (Polychaeta, Nereididae) has been recognized as a slow-evolving lophotrochozoan that attracts increasing attention as a valuable model for evolutionary and developmental research. Here, we analyzed its immune-related transcriptome. For targeted identification of immune-induced genes we injected bacterial lipopolysaccharide, a commonly used elicitor of innate immune responses, and applied the suppression subtractive hybridization technique that selectively amplifies cDNAs of differentially expressed genes.

Results

Sequence analysis of 288 cDNAs revealed induced expression of numerous genes whose potential homologues from other animals mediate recognition of infection (e.g. complement receptor CD35), signaling (e.g. myc and S

SUBMITTER: Altincicek B 

PROVIDER: S-EPMC1939704 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature