Enzymatic engineering of the porcine genome with transposons and recombinases.

ABSTRACT:

Background

Swine is an important agricultural commodity and biomedical model. Manipulation of the pig genome provides opportunity to improve production efficiency, enhance disease resistance, and add value to swine products. Genetic engineering can also expand the utility of pigs for modeling human disease, developing clinical treatment methodologies, or donating tissues for xenotransplantation. Realizing the full potential of pig genetic engineering requires translation of the complete repertoire of genetic tools currently employed in smaller model organisms to practical use in pigs.

Results

Application of transposon and recombinase technologies for manipulation of the swine genome requires characterization of their activity in pig cells. We tested four transposon systems-

SUBMITTER: Clark KJ 

PROVIDER: S-EPMC1939997 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature