Quantitative promoter methylation analysis of multiple cancer-related genes in renal cell tumors.

ABSTRACT:

Background

Aberrant promoter hypermethylation of cancer-associated genes occurs frequently during carcinogenesis and may serve as a cancer biomarker. In this study we aimed at defining a quantitative gene promoter methylation panel that might identify the most prevalent types of renal cell tumors.

Methods

A panel of 18 gene promoters was assessed by quantitative methylation-specific PCR (QMSP) in 85 primarily resected renal tumors representing the four major histologic subtypes (52 clear cell (ccRCC), 13 papillary (pRCC), 10 chromophobe (chRCC), and 10 oncocytomas) and 62 paired normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters.

Results

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SUBMITTER: Costa VL 

PROVIDER: S-EPMC1940017 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature