Precise protein quantification based on peptide quantification using iTRAQ.

ABSTRACT:

Background

Mass spectrometry based quantification of peptides can be performed using the iTRAQ reagent in conjunction with mass spectrometry. This technology yields information about the relative abundance of single peptides. A method for the calculation of reliable quantification information is required in order to obtain biologically relevant data at the protein expression level.

Results

A method comprising sound error estimation and statistical methods is presented that allows precise abundance analysis plus error calculation at the peptide as well as at the protein level. This yields the relevant information that is required for quantitative proteomics. Comparing the performance of our method named Quant with existing approaches the error estimation is reliable and offer

SUBMITTER: Boehm AM 

PROVIDER: S-EPMC1940031 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature