A procedure for the prediction of temperature-sensitive mutants of a globular protein based solely on the amino acid sequence.

ABSTRACT: Temperature-sensitive (Ts) mutants of a protein are an extremely powerful tool for studying protein function in vivo and in cell culture. We have devised a method to predict those residues in a protein sequence that, when appropriately mutated, are most likely to give rise to a Ts phenotype. Since substitutions of buried hydrophobic residues often result in significant destabilization of the protein, our method predicts those residues in the sequence that are likely to be buried in the protein structure. We also indicate a set of amino acid substitutions, which should be made to generate a Ts mutant of the protein. This method requires only the protein sequence. No structural information or homologous sequence information is required. This method was applied to a test data set of 30 nonhom

SUBMITTER: Varadarajan R 

PROVIDER: S-EPMC19465 | biostudies-literature | 1996 Nov

REPOSITORIES: biostudies-literature