Molecular characterization of a novel intracellular ADP-ribosyl cyclase.

ABSTRACT:

Background

ADP-ribosyl cyclases are remarkable enzymes capable of catalyzing multiple reactions including the synthesis of the novel and potent intracellular calcium mobilizing messengers, cyclic ADP-ribose and NAADP. Not all ADP-ribosyl cyclases however have been characterized at the molecular level. Moreover, those that have are located predominately at the outer cell surface and thus away from their cytosolic substrates.

Methodology/principal findings

Here we report the molecular cloning of a novel expanded family of ADP-ribosyl cyclases from the sea urchin, an extensively used model organism for the study of inositol trisphosphate-independent calcium mobilization. We provide evidence that one of the isoforms (SpARC1) is a soluble protein that is targeted exclusively to t

SUBMITTER: Churamani D 

PROVIDER: S-EPMC1949048 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature