Replication fork velocities at adjacent replication origins are coordinately modified during DNA replication in human cells.

ABSTRACT: The spatial organization of replicons into clusters is believed to be of critical importance for genome duplication in higher eukaryotes, but its functional organization still remains to be fully clarified. The coordinated activation of origins is insufficient on its own to account for a timely completion of genome duplication when interorigin distances vary significantly and fork velocities are constant. Mechanisms coordinating origin distribution with fork progression are still poorly elucidated, because of technical difficulties of visualizing the process. Taking advantage of a single molecule approach, we delineated and compared the DNA replication kinetics at the genome level in human normal primary and malignant cells. Our results show that replication forks moving from one origin, a

SUBMITTER: Conti C 

PROVIDER: S-EPMC1949372 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature