Bacillus anthracis secretome time course under host-simulated conditions and identification of immunogenic proteins.

ABSTRACT:

Background

The secretion time course of Bacillus anthracis strain RA3R (pXO1+/pXO2-) during early, mid, and late log phase were investigated under conditions that simulate those encountered in the host. All of the identified proteins were analyzed by different software algorithms to characterize their predicted mode of secretion and cellular localization. In addition, immunogenic proteins were identified using sera from humans with cutaneous anthrax.

Results

A total of 275 extracellular proteins were identified by a combination of LC MS/MS and MALDI-TOF MS. All of the identified proteins were analyzed by SignalP, SecretomeP, PSORT, LipoP, TMHMM, and PROSITE to characterize their predicted mode of secretion, cellular localization, and protein domains. Fifty-three proteins wer

SUBMITTER: Walz A 

PROVIDER: S-EPMC1949399 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature