The Pseudomonas putida Lon protease is involved in N-acyl homoserine lactone quorum sensing regulation.

ABSTRACT:

Background

In Pseudomonas putida and Pseduomonas aeruginosa, the similar PpuR/RsaL/PpuI and LasR/RsaL/LasI acyl homoserine lactones (AHLs) quorum sensing (QS) systems have been shown to be under considerable regulation by other global regulators. A major regulator is the RsaL protein which strongly directly represses the transcription of the P. putida ppuI and P. aeruginosa lasI AHL synthases. In this study we screened a transposon mutant bank of P. putida in order to identify if any other regulators were involved in negative regulation of AHL QS.

Results

In our screen we identified three Tn5 mutants which displayed overproduction of AHLs in P. putida strain WCS358. Two of the mutants had a Tn5 located in the rsaL gene, whereas in one mutant the transposon was located in the

SUBMITTER: Bertani I 

PROVIDER: S-EPMC1949823 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature