Use of tiling array data and RNA secondary structure predictions to identify noncoding RNA genes.

ABSTRACT:

Background

Within the last decade a large number of noncoding RNA genes have been identified, but this may only be the tip of the iceberg. Using comparative genomics a large number of sequences that have signals concordant with conserved RNA secondary structures have been discovered in the human genome. Moreover, genome wide transcription profiling with tiling arrays indicate that the majority of the genome is transcribed.

Results

We have combined tiling array data with genome wide structural RNA predictions to search for novel noncoding and structural RNA genes that are expressed in the human neuroblastoma cell line SK-N-AS. Using this strategy, we identify thousands of human candidate RNA genes. To further verify the expression of these genes, we focused on candidate genes

SUBMITTER: Weile C 

PROVIDER: S-EPMC1949828 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature