Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets.

ABSTRACT:

Background

To gain insight into host-microbe interactions in a piglet model, a functional genomics approach was used to address the working hypothesis that transcriptionally regulated genes associated with promoting epithelial barrier function are activated as a defensive response to the intestinal microbiota. Cesarean-derived germfree (GF) newborn piglets were colonized with adult swine feces, and villus and crypt epithelial cell transcriptomes from colonized and GF neonatal piglets were compared using laser-capture microdissection and high-density porcine oligonucleotide microarray technology.

Results

Consistent with our hypothesis, resident microbiota induced the expression of genes contributing to intestinal epithelial cell turnover, mucus biosynthesis, and priming of th

SUBMITTER: Chowdhury SR 

PROVIDER: S-EPMC1949829 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature