Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins.

ABSTRACT:

Background

Expression of higher eukaryotic genes as soluble, stable recombinant proteins is still a bottleneck step in biochemical and structural studies of novel proteins today. Correct identification of stable domains/fragments within the open reading frame (ORF), combined with proper cloning strategies, can greatly enhance the success rate when higher eukaryotic proteins are expressed as these domains/fragments. Furthermore, a HTP cloning pipeline incorporated with bioinformatics domain/fragment selection methods will be beneficial to studies of structure and function genomics/proteomics.

Results

With bioinformatics tools, we developed a domain/domain boundary prediction (DDBP) method, which was trained by available experimental data. Combined with an improved cloning str

SUBMITTER: Chen Y 

PROVIDER: S-EPMC1950093 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature