Wingless/Wnt signal transduction requires distinct initiation and amplification steps that both depend on Arrow/LRP.

ABSTRACT: Members of the Wg/Wnt family provide key intercellular signals during embryonic development and in the maintenance of homeostatic processes, but critical aspects of their signal transduction pathways remain controversial. We have found that canonical Wg signaling in Drosophila involves distinct initiation and amplification steps, both of which require Arrow/LRP. Expressing a chimeric Frizzled2-Arrow protein in flies that lack endogenous Wg or Arrow showed that this construct functions as an activated Wg receptor but is deficient in signal amplification. In contrast, a chimeric Arrow protein containing the dimerization domain of Torso acted as a potent amplifier of Wg signaling but could not initiate Wg signaling on its own. The two chimeric proteins synergized, so that their co-expression

SUBMITTER: Baig-Lewis S 

PROVIDER: S-EPMC1950126 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature