An analysis of expressed sequence tags of developing castor endosperm using a full-length cDNA library.

ABSTRACT:

Background

Castor seeds are a major source for ricinoleate, an important industrial raw material. Genomics studies of castor plant will provide critical information for understanding seed metabolism, for effectively engineering ricinoleate production in transgenic oilseeds, or for genetically improving castor plants by eliminating toxic and allergic proteins in seeds.

Results

Full-length cDNAs are useful resources in annotating genes and in providing functional analysis of genes and their products. We constructed a full-length cDNA library from developing castor endosperm, and obtained 4,720 ESTs from 5'-ends of the cDNA clones representing 1,908 unique sequences. The most abundant transcripts are genes encoding storage proteins, ricin, agglutinin and oleosins. Several other

SUBMITTER: Lu C 

PROVIDER: S-EPMC1950504 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature