Real-time monitoring of functional interactions between upstream and core promoter sequences in living cells of sea urchin embryos.

ABSTRACT: There are some functional compatibilities between upstream and core promoter sequences for transcriptional activation in yeast, Drosophila and mammalian cells. Here we examined whether similar compatibilities exist in sea urchin embryos, and if so, whether they are dynamically regulated during early development. Two reporter plasmids, each containing a test promoter conjugated to either CFP or YFP, were concurrently introduced into embryos, and their expression patterns were studied by fluorescence microscopy. The upstream sequence of the Hemicentrotus pulcherrimus (Hp) OtxE promoter drives the expression of its own core promoter and that of Strongylocentrotus purpuratus (Sp) Spec2a in different embryonic regions, especially at the late gastrula stage. Interestingly, when the four putative

SUBMITTER: Kobayashi A 

PROVIDER: S-EPMC1950538 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature