Global changes in optic nerve head gene expression after exposure to elevated intraocular pressure in a rat glaucoma model.

ABSTRACT:

Purpose

In glaucoma, the optic nerve head (ONH) is the likely site of initial injury and elevated intraocular pressure (IOP) is the best-known risk factor. This study determines global gene expression changes in the pressure-injured ONH.

Methods

Unilateral sustained IOP elevation (glaucoma, n = 46) or optic nerve transection (n = 10) was produced in rats. ONHs were removed, and the retrobulbar optic nerves were graded for degeneration. Gene expression in the glaucomatous ONH with extensive injury was compared with that in the fellow ONH (n = 6/group), by using cDNA microarrays. Data from 12 arrays were normalized, significant differences in gene expression determined, and significantly affected gene classes identified. For the remaining ONH, grouped by experimental condition

SUBMITTER: Johnson EC 

PROVIDER: S-EPMC1950563 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature