The TATA-binding protein regulates maternal mRNA degradation and differential zygotic transcription in zebrafish.

ABSTRACT: Early steps of embryo development are directed by maternal gene products and trace levels of zygotic gene activity in vertebrates. A major activation of zygotic transcription occurs together with degradation of maternal mRNAs during the midblastula transition in several vertebrate systems. How these processes are regulated in preparation for the onset of differentiation in the vertebrate embryo is mostly unknown. Here, we studied the function of TATA-binding protein (TBP) by knock down and DNA microarray analysis of gene expression in early embryo development. We show that a subset of polymerase II-transcribed genes with ontogenic stage-dependent regulation requires TBP for their zygotic activation. TBP is also required for limiting the activation of genes during development. We reveal tha

SUBMITTER: Ferg M 

PROVIDER: S-EPMC1950726 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature