Transcription termination defective mutants of Rho: role of different functions of Rho in releasing RNA from the elongation complex.

ABSTRACT: The transcription termination factor Rho of Escherichia coli is a RNA binding protein which can translocate along the RNA and unwind the RNA:DNA hybrid using the RNA-dependent ATPase activity. In order to investigate the involvement of each of these functions in releasing RNA from the elongation complex, we have isolated different termination defective mutants of Rho by random mutagenesis, characterized them for their different functions and established the structure-function correlations from the available structural data of Rho. These mutations are located within the two domains; the N-terminal RNA binding domain (G51V, G53V, and Y80C) and in the C-terminal ATP binding domain (Y274D, P279S, P279L, G324D, N340S, I382N) including the two important structural elements, the Q-loop (P279S, P2

SUBMITTER: Chalissery J 

PROVIDER: S-EPMC1950744 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature