A systematic screen for genes expressed in definitive endoderm by Serial Analysis of Gene Expression (SAGE).

ABSTRACT:

Background

The embryonic definitive endoderm (DE) gives rise to organs of the gastrointestinal and respiratory tract including the liver, pancreas and epithelia of the lung and colon. Understanding how DE progenitor cells generate these tissues is critical to understanding the cause of visceral organ disorders and cancers, and will ultimately lead to novel therapies including tissue and organ regeneration. However, investigation into the molecular mechanisms of DE differentiation has been hindered by the lack of early DE-specific markers.

Results

We describe the identification of novel as well as known genes that are expressed in DE using Serial Analysis of Gene Expression (SAGE). We generated and analyzed three longSAGE libraries from early DE of murine embryos: early whole

SUBMITTER: Hou J 

PROVIDER: S-EPMC1950885 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature