"Mark the gene": a method for nondestructive introduction of marker sequences inside the gene frame of transgenes.

ABSTRACT: A specific marking and detection technique is a fundamental requirement for the safer use of genetically modified (GM) organisms. Here we propose a simple and effective method for directly marking functional transgenes in GM organisms. For that purpose, we introduced nucleotide substitutions (NS), based on the degeneracy of codons as markers (NS markers), into the bphC (2,3-dihydroxybiphenyl dioxygenase) and tomA3 (toluene-ortho-monooxygenase) gene frames using a PCR-based method. No change was observed in the enzyme activity of translated proteins, and alignments with homologous genes showed the uniqueness of the NS markers. Furthermore, we constructed tomA3 variations harboring NS markers in different positions. Although the translational products were identical, the constructed variatio

SUBMITTER: Morono Y 

PROVIDER: S-EPMC1951010 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature