SigmaB and the sigmaB-dependent arlRS and yabJ-spoVG loci affect capsule formation in Staphylococcus aureus.

ABSTRACT: The alternative transcription factor sigma(B) of Staphylococcus aureus affects the transcription of the cap gene cluster, required for the synthesis of capsular polysaccharide (CP), although this operon is lacking an apparent sigma(B)-dependent promoter. Regulation of cap expression and CP production in S. aureus strain Newman was shown here to be influenced by sigma(B), the two-component signal transduction regulatory system ArlRS, and the yabJ-spoVG locus to different extents. Inactivation of arlR or deletion of the sigB operon strongly suppressed capA (CP synthesis enzyme A) transcription. Deletion of spoVG had a polar effect on yabJ-spoVG transcription and resulted in a two- to threefold decrease in capA transcription. Interestingly, immunofluorescence showed that CP production was str

SUBMITTER: Meier S 

PROVIDER: S-EPMC1951174 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature