Transcriptome analysis in response to heat shock and cadmium in the aquatic fungus Blastocladiella emersonii.

ABSTRACT: The global transcriptional response of the chytridiomycete Blastocladiella emersonii to environmental stress conditions was explored by sequencing a large number of expressed sequence tags (ESTs) from three distinct cDNA libraries, constructed with mRNA extracted from cells exposed to heat shock and different concentrations of cadmium chloride. A total of 6,350 high-quality EST sequences were obtained and assembled into 2,326 putative unigenes, 51% of them not previously described in B. emersonii. To approximately 59% of the unigenes it was possible to assign an orthologue in another organism, whereas 41% of them remained without a putative identification, with transcripts related to protein folding and antioxidant activity being highly enriched in the stress libraries. A microarray chip w

SUBMITTER: Georg RC 

PROVIDER: S-EPMC1951522 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature