Target genes and DNA-binding sites of the response regulator PhoR from Corynebacterium glutamicum.

ABSTRACT: The two-component signal transduction system PhoRS of Corynebacterium glutamicum is involved in the phosphate (P(i)) starvation response. To analyze the binding of unphosphorylated and phosphorylated PhoR to the promoters of phosphate starvation-inducible (psi) genes, this response regulator and the kinase domain of its cognate sensor, PhoS (MBP-PhoSDelta1-246), were overproduced and purified. MBP-PhoSDelta1-246 showed constitutive autophosphorylation activity, and a rapid phosphoryl group transfer from phosphorylated MBP-PhoSDelta1-246 to PhoR was observed. Gel mobility shift assays revealed that phosphorylation increases the DNA-binding affinity of PhoR. The affinity of PhoR approximately P to different promoters varied and decreased in the order pstSCAB > phoRS > phoC > ushA > porB > ug

SUBMITTER: Schaaf S 

PROVIDER: S-EPMC1951857 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature