Mutational analysis of the ompA promoter from Flavobacterium johnsoniae.

ABSTRACT: Sequences that mediate the initiation of transcription in Flavobacterium species are not well known. The majority of identified Flavobacterium promoter elements show homology to those of other members of the phylum Bacteroidetes, but not of proteobacteria, and they function poorly in Escherichia coli. In order to analyze the Flavobacterium promoter structure systematically, we investigated the -33 consensus element, -7 consensus element, and spacer length of the Flavobacterium ompA promoter by measuring the effects of site-directed mutations on promoter activity. The nonconserved sequences in the spacer region and in regions close to the consensus motifs were randomized in order to determine their importance for promoter activity. Most of the base substitutions in these regions caused larg

SUBMITTER: Chen S 

PROVIDER: S-EPMC1951883 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature