Functional characterization of the Sinorhizobium meliloti acetate metabolism genes aceA, SMc00767, and glcB.

ABSTRACT: The genes encoding malate synthase (glcB) and isocitrate lyase (aceA) and a 240-bp open reading frame (SMc00767) located downstream of aceA were isolated and functionally characterized in Sinorhizobium meliloti. Independent and double interposon mutants of each gene were constructed, and the corresponding phenotypes were analyzed. aceA mutants failed to grow on acetate, and mutants deficient in SMc00767 were also affected in acetate utilization. In contrast, mutants deficient in glcB grew on acetate similar to wild-type strain Rm5000. Complementation experiments showed that aceA and SMc00767 gene constructs were able to restore the growth on acetate in the corresponding single mutants. aceA-glcB, aceA-SMc00767, and glcB-SMc00767 double knockouts were also unable to grow on acetate, but thi

SUBMITTER: Ramirez-Trujillo JA 

PROVIDER: S-EPMC1952029 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature