The 90S preribosome is a multimodular structure that is assembled through a hierarchical mechanism.

ABSTRACT: The 90S preribosomal particle is required for the production of the 18S rRNA from a pre-rRNA precursor. Despite the identification of the protein components of this particle, its mechanism of assembly and structural design remain unknown. In this work, we have combined biochemical studies, proteomic techniques, and bioinformatic analyses to shed light into the rules of assembly of the yeast 90S preribosome. Our results indicate that several protein subcomplexes work as discrete assembly subunits that bind in defined steps to the 35S pre-rRNA. The assembly of the t-UTP subunit is an essential step for the engagement of at least five additional subunits in two separate, and mutually independent, assembling routes. One of these routes leads to the formation of an assembly intermediate compose

SUBMITTER: Perez-Fernandez J 

PROVIDER: S-EPMC1952102 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature