Divide and conquer: enriching environmental sequencing data.

ABSTRACT:

Background

In environmental sequencing projects, a mix of DNA from a whole microbial community is fragmented and sequenced, with one of the possible goals being to reconstruct partial or complete genomes of members of the community. In communities with high diversity of species, a significant proportion of the sequences do not overlap any other fragment in the sample. This problem will arise not only in situations with a relatively even distribution of many species, but also when the community in a particular environment is routinely dominated by the same few species. In the former case, no genomes may be assembled at all, while in the latter case a few dominant species in an environment will always be sequenced at high coverage to the detriment of coverage of the greater number of

SUBMITTER: Bergeron A 

PROVIDER: S-EPMC1952108 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature